PHYTOCHEMICAL AND IN VITRO ANTIOXIDANT EVALUATION OF PERICALLIS CRUENTA (L HER.) BOLLE

Abstract

The present study deals with the phytochemical screening and In Vitro antioxidant activities of leaves extract of Pericallis cruenta. The methanolic extract of the leaves was prepared by maceration. Different tests were performed to find out the phytochemicals present in the leaves. Five assays i.e. DPPH Radical Scavenging Activity, Ferric Reducing Antioxidant Power (FRAP), Total Flavonoid Content (TFC), Total Antioxidant Activity (TAA) and Metal Chelating Activity were used to assess the antioxidant activity. The phytochemical screening exposed the existence of saponins, proteins & amino acids, carbohydrates, phenols & tannins, flavonoids and sterols. DPPH Radical Scavenging Activity was calculated for different concentrations i.e. 1ml, 0.75ml, 0.50ml and 0.25ml. It was maximum for 0.25ml concentration i.e. 92.06% and lowest as 48.83% for 1ml concentration. Trolox Equivalent (TE) for FRAP was calculated as 4.52 μM/ml. The value for Total Antioxidant Activity (TAA) was found to be 108.5mg/ml. Moreover, value for Total Flavonoid Content (TFC) was 40.8 mg Ru./g. The percentage iron bound value for Metal Chelating Activity was very high and found to be 1211.20%. This high value indicates that the leaves of P. cruenta are a source of potential antioxidants. Therefore, this plant is recommended for further study to find out its use for the treatment of diseases.